Part:BBa_K1114206:Design
CviR_CD
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 44
Illegal NgoMIV site found at 571
Illegal AgeI site found at 325 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 338
Design Notes
This is the MoClo formatted part of CviR with CD fusion sites. This is a Level 0 MoClo part with flanking sites C on the 5' side and site D on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. Summary of modifications from original part: Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See pSB1C3.
Source
Holden Lab
References
[1] Stauff, D.L., and Bassler, B.L. (2011) "Quorum Sensing in Chromobacterium violaceum: DNA Recognition and Gene Regulation by the CviR Receptor." Journal of Bacteriology 193(15):3871-3878. doi: 10.1128/JB.05125-11.